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sterile biomasher (TaKaRa)

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TaKaRa sterile biomasher
Sterile Biomasher, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biomasher+standard/TaKaRa+BioMasher+Standard/pm42098131-393-19-22
Average 95 stars, based on 83 article reviews

sterile biomasher - by Bioz Stars, 2026-09

95/100 stars

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Homogenization:

Article Title: Disruptions in hepatic glucose metabolism are involved in the diminished efficacy after chronic treatment with glucokinase activator
Article Snippet: .. The liver was homogenized at 4°C in Takara Biomasher Standard (Takara Bio INC, Shiga, Japan) containing homogenization buffer (50 mmol/L HEPES, 5 mmol/L MgCl 2 , 100 mmol/L KCl, 1 mmol/L EDTA, 1% triton-X, Protease inhibitor cocktail (Sigma-Aldrich, MO, USA)). ..

Protease Inhibitor:

Isolation:

Article Title: Humanized substitutions of Vmat1 in mice alter amygdala-dependent behaviors associated with the evolution of anxiety
Article Snippet: .. The extracted tissues were homogenized in BioMasher Standard (TaKaRa, Shiga, Japan) and total RNA was isolated using the RNeasy® Plus Mini Kit (Qiagen, Hilden, Germany). .. RNA concentration and purity were assessed using a Nano-Drop® ND-1000 spectrophotometer (Thermo Scientific, Waltham, MA), and total RNA integrity was quantified by the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).

Article Title: Genomic adaptive potential to cold environments in the invasive red swamp crayfish
Article Snippet: .. Tissues were homogenized in BioMasher Standard (TaKaRa, Shiga, Japan), and total RNA was isolated using a Maxwell 16 LEV Plant RNA Kit (Promega, Madison, WI). .. RNA concentration and purity were assessed using a Nano-Drop® ND-2000 spectrophotometer (Thermo Scientific, Waltham, MA), and total RNA integrity was quantified using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).

Olfactory:

Article Title: Repeated exposure to kairomone-containing coffee odor improves abnormal olfactory behaviors in heterozygous oxytocin receptor knock-in mice
Article Snippet: Thereafter, the dissected mouse brains were soaked in 5 ml of RNA later solution (Invitrogen) for 24 h at 4°C and preserved at –80°C. .. To collect mRNA from these samples, the preserved brains were defrosted at ambient temperature; thereafter, the brain samples were obtained in the same manner as previously described ( ) and also confirmed by the mouse brain atlas , that is, approximately 10 mg of the hypothalamus, olfactory bulb, and amygdala tissues were excised, placed in tubes, and homogenized by BioMasher Standard (Takara, Japan). .. The mRNA was extracted as follows: The mashed brain samples were used to purify RNAs using an RNeasy Plus Micro kit (Qiagen). cDNAs were generated by Reverse Transcription with the PrimeScriptTM kit (Takara).

Purification:

Article Title: Increased expression of acyl-CoA oxidase 2 in the kidney with plasma phytanic acid and altered gut microbiota in spontaneously hypertensive rats.
Article Snippet: We performed a DNA microarray analysis of the renal medulla and cortex from spontaneously hypertensive rats (SHRs), stroke-prone SHRs (SHRSPs), and Wistar–Kyoto (WKY) rats to identify pivotal molecules in the kidney associated with the onset of hypertension and found increased expression of acyl-CoA oxidase 2 (Acox2) mRNA.. Real-time polymerase chain reaction revealed that Acox2 mRNA expression in the renal medulla and cortex of SHRs and SHRSPs was increased in comparison to WKY rats.. These findings indicate that increased renal ACOX2 (an enzyme that induces the β-oxidation of fatty acids) is associated with the onset of hypertension.